Purification and characterization of glycolic acid oxidase from pig liver
نویسندگان
چکیده
منابع مشابه
Effect of anions on the catalytic activity of pig liver glycolic acid oxidase.
I. Pig liver glycolic acid oxidase (glycolate: 02 oxidoreductase, EC 1.1.3.1 ) can function with either 2,6-dichlorophenolindophenol (DCIP) (Vmax----125o moles of glycolate per mole of ravin per rain) or 02 (Vmax = 620 moles of glycolate per mole of ravin per rain)9,1° as the electron acceptor. 2. Straight-chain monocarboxylic acids are non-competitive inhibitors of this enzyme with either glyc...
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In mammalian tissues cardiolipin is rapidly remodeled by monolysocardiolipin acyltransferase subsequent to its de novo biosynthesis (Ma, B. J., Taylor, W. A, Dolinsky, V. W., and Hatch, G. M. (1999) J. Lipid Res. 40, 1837-1845). We report here the purification and characterization of a monolysocardiolipin acyltransferase activity from pig liver mitochondria. Monolysocardiolipin acyltransferase ...
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A procedure for the purification of the enzyme bile acid:CoA ligase from guinea pig liver microsomes was developed. Activity toward chenodeoxycholate, cholate, deoxycholate, and lithocholate co-purified suggesting that a single enzyme form catalyzes the activation of all four bile acids. Activity toward lithocholate could not be accurately assayed during the earlier stages of purification due t...
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The enzyme which oxidizes glycolic acid to glyoxylic acid is present in sufficient concentrations to support active respiration in a wide variety of plants (1). Glycolic acid itself is present in plants in small amounts (2), and, as yet, it has been the only 2-carbon organic acid which has been found to accumulate Cl4 during short time photosynthesis experiments with Cl402 (3). Thus both the fr...
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Flavonoids could serve as potent inhibitors of xanthine oxidase (XO). In the present study, the effects of Ruta graveolens L. extract and its major isolated flavonoids, quercetin and rutin, on guinea pig liver XO have been investigated. The inhibitory effects of R. graveolens, quercetin and its glycoside form, rutin, were assayed spectrophotometrically. R. graveolens...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Enzymology
سال: 1971
ISSN: 0005-2744
DOI: 10.1016/0005-2744(71)90003-9